Licht-im-Terrarium: Literaturdatenbank
Licht-im-Terrarium: Literaturdatenbank |
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Jones, G., & Kaufmann, M. (2016). Vitamin d metabolite profiling using liquid chromatography–tandem mass spectrometry (lc–ms/ms). The Journal of Steroid Biochemistry and Molecular Biology, 164, 110–114. Added by: Sarina (2022-08-05 13:06:12) Last edited by: Sarina (2022-09-26 12:37:43) |
| Resource type: Journal Article DOI: https://doi.org/10.1016/j.jsbmb.2015.09.026 ID no. (ISBN etc.): 0960-0760 BibTeX citation key: Jones2016 View all bibliographic details  |
Categories: Englisch = English Keywords: Vitamin D = Vitamin D Creators: Jones, Kaufmann Collection: The Journal of Steroid Biochemistry and Molecular Biology |
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| Abstract |
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Liquid chromatography tandem mass spectrometry (LC–MS/MS) has emerged as the latest technology to be used to assay the metabolites of vitamin D. The method uses molecular mass as a detection technique after straightforward extraction and chromatography steps. LC–MS/MS assay provides a level of accuracy and reproducibility not seen before with other methods and is beginning to rival antibody-based methods in terms of sensitivity and convenience. Methods for detection of underivatized and DMEQ-TAD derivatized vitamin D metabolites are evaluated. Sensitivity is improved by 10–100 fold with derivatization and allows for the simultaneous assay of multiple vitamin D metabolites, a process termed vitamin D metabolite profiling. Clinical and research applications of vitamin D metabolite profiling are discussed.
Added by: Sarina |