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Selleri, P. & Di Girolamo, N. (2012) Plasma 25-hydroxyvitamin D3 concentrations in Hermann’s tortoises (Testudo hermanni) exposed to natural sunlight and two artificial ultraviolet radiation sources. American journal of veterinary research, 73 1781–1786. 
Added by: Sarina (19 Nov 2012 09:26:56 UTC)   Last edited by: Sarina (11 Nov 2014 18:54:03 UTC)
Resource type: Journal Article
DOI: 10.2460/ajvr.73.11.1781
BibTeX citation key: Selleri2012
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Categories: Englisch = English
Keywords: Schildkröten = Turtles, Ultraviolett = Ultraviolet, Vitamin D = Vitamin D
Creators: Di Girolamo, Selleri
Collection: American journal of veterinary research
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Abstract
Objective—To determine the effect of various UVB radiation sources on plasma 25-hydroxyvitamin D3 concentrations in Hermann's tortoises (Testudo hermanni).

Animals—18 healthy Hermann's tortoises.

Procedures—Tortoises were exposed to sunlight in an outdoor enclosure located in the natural geographic range of Hermann's tortoises (n = 6 tortoises) or a self-ballasted mercury-vapor lamp (6) or fluorescent UVB-emitting lamp (6) in an indoor enclosure for 35 days. Plasma samples were obtained from each tortoise on the first (day 0) and last (day 35) days of the study, and concentrations of 25-hydroxyvitamin D3 were determined. Amount of UVB radiation in enclosures was measured.

Results—Mean ± SD plasma 25-hydroxyvitamin D3 concentrations for tortoises exposed to the mercury-vapor and fluorescent lamps were significantly lower on day 35 (155.69 ± 80.71 nmol/L and 134.42 ± 51.42 nmol/L, respectively) than they were on day 0 (368.02 ± 119.34 nmol/L and 313.69 ± 109.54 nmol/L, respectively). Mean ± SD plasma 25-hydroxyvitamin D3 concentration for tortoises exposed to sunlight did not differ significantly between days 0 (387.74 ± 114.56 nmol/L) and 35 (411.51 ± 189.75 nmol/L). Mean day 35 plasma 25-hydroxyvitamin D3 concentration was significantly higher for tortoises exposed to sunlight versus those exposed to mercury-vapor or fluorescent lamps. Sunlight provided significantly more UVB radiation than did the mercury-vapor or fluorescent lamps.

Conclusions and Clinical Relevance—Plasma 25-hydroxyvitamin D3 concentrations differed between tortoises exposed to sunlight and those exposed to artificial UVB sources. Exposure to sunlight at a latitude similar to that of the natural geographic range is recommended for healthy and calcium-deficient tortoises.
Added by: Sarina  Last edited by: Sarina
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