Licht-im-Terrarium: Literaturdatenbank
Licht-im-Terrarium: Literaturdatenbank |
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Wicks, N. L., Chan, J. W., Najera, J. A., Ciriello, J. M., & Oancea, E. (2011). Uva phototransduction drives early melanin synthesis in human melanocytes. Current Biology, 21, Added by: Sarina (2011-11-09 11:38:58) |
| Resource type: Journal Article DOI: 10.1016/j.cub.2011.09.047 BibTeX citation key: Wicks2011 View all bibliographic details  |
Categories: Englisch = English Keywords: Ultraviolett = Ultraviolet, Vitamin D = Vitamin D Creators: Chan, Ciriello, Najera, Oancea, Wicks Collection: Current Biology |
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| Abstract |
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Exposure of human skin to solar ultraviolet radiation (UVR), a powerful carcinogen [1] comprising w95% ultraviolet A (UVA) and w5% ultraviolet B (UVB) at the Earth’s surface, promotes melanin synthesis in epidermal melanocytes [2, 3], which protects skin from DNA damage [4, 5]. UVB causes DNA lesions [6] that lead to transcriptional activation of melanin-producing enzymes, resulting in delayed skin pigmentation within days [7]. In contrast, UVA causes primarily oxidative damage [8] and leads to immediate pigment darkening (IPD) within minutes, via an unknown mechanism [9, 10]. No receptor protein directly mediating phototransduction in skin has been identified. Here we demonstrate that exposure of primary human epidermal melanocytes (HEMs) to UVA causes calcium mobilization and early melanin synthesis. Calcium responses were abolished by treatment with G protein or phospholipase C (PLC) inhibitors or by depletion of intracellular calcium stores. We show that the visual photopigment rhodopsin [11] is expressed in HEMs and contributes to UVR phototransduction. Upon UVR exposure, significant melanin production was measured within one hour; cellular melanin continued to increase in a retinal- and calcium-dependent manner up to 5-fold after 24 hr. Our findings identify a novel UVA-sensitive signaling pathway in melanocytes that leads to calcium mobilization and melanin synthesis and may underlie the mechanism of IPD in human skin. ############################## http://www.sciencedaily.com/releases/2011/11/111103132245.htm Skin 'Sees' UV Light, Starts Producing Pigment ScienceDaily (Nov. 3, 2011) — In a new study, biologists report that melanocyte skin cells detect ultraviolet light using a photosensitive receptor previously thought to exist only in the eye. This eye-like ability of skin to sense light triggers the production of melanin within hours, more quickly than previously thought, in an apparent rush to protect against damage to DNA. For most people, tanning seems a simple proposition. A naturally light-skinned person lies in the sun for hours and ends up as bronzed as a Jersey Shore star. To scientists, the reaction of skin to ultraviolet light is more mysterious. A new study demonstrates that skin detects UVA radiation using a light-sensitive receptor previously found only in the eye and that this starts melanin production within a couple of hours. Until now, scientists only knew that melanin production occurred days after UVB radiation had already begun damaging DNA. "As soon as you step out into the sun, your skin knows that it is exposed to UV radiation," said senior author Elena Oancea, assistant professor of biology in the Department of Molecular Pharmacology, Physiology, and Biotechnology at Brown University. "This is a very fast process, faster than anything that was known before." Scientists believe that melanin protects the DNA in skin cells against damage from UVB rays by absorbing the incoming radiation. It isn't perfect, which is why people must use sun block. But the new study in the journal Current Biology shows that the body mounts its defense much sooner, well before it becomes apparent in the form of a tan. In lab experiments with human melanin-producing skin cells called melanocytes, Oancea, graduate student Nadine Wicks, and their team discovered that the cells contain rhodopsin, a photosensitive receptor used by the eye to detect light. Moreover, they traced the steps of how rhodopsin unleashes calcium ion signals that instigate melanin production. Eyes on the skin In the team's first experiment, the scientists were looking to see whether UV light instigated a calcium signaling response. They found nothing. But guessing that the skin might sense light like the eyes do, they added retinal, a co-factor of opsin receptors including rhodopsin. "When we did that, we saw an immediate and massive calcium response," said Wicks, the study's lead author. Further investigations found that the cells contained rhodopsin RNA and protein. Under UV light, when the scientists reduced rhodopsin levels in the cells, calcium signaling was reduced. Later, when they starved cells of retinal, they found that melanin production dropped. The authors also determined that long-wavelength UVA light, rather than short-wavelength UVB light, is what stimulates rhodopsin in melanocytes. During several experiments they were able to trace the following process: When UVA light strikes rhodopsin receptors with retinal, calcium signals are triggered within a few seconds. After an hour, measurable amounts of melanin accumulate, although in relatively small quantities compared to the production that will occur within 24 hours. As much as they learned, Oancea and Wicks still have some questions. One is whether rhodopsin is acting alone or in concert with another yet undiscovered receptor. Another question is whether melanocytes immediately begin exporting melanin to other kinds of skin cells for protection or whether they keep the early supply for themselves. Just because scientists are learning more about how the skin responds to and protects itself against UV radiation, Oancea said, that's no reason for people to change what they do to protect themselves. "This doesn't say, 'Don't use sunscreen'," Oancea said. In addition to Oancea and Wicks, other authors were Jason Chan, Julia Najera, and Jonathan Ciriello. Brown University, the National Institutes of Health, and the Natural Sciences and Engineering Research Council of Canada supported the research. Added by: Sarina Last edited by: Sarina |