Licht-im-Terrarium: Literaturdatenbank
Licht-im-Terrarium: Literaturdatenbank |
|
 |
|
Singh, R. J., Taylor, R. L., Reddy, S. G., & Grebe, S. K. G. (2006). C-3 Epimers Can Account for a Significant Proportion of Total Circulating 25-Hydroxyvitamin D in Infants, Complicating Accurate Measurement and Interpretation of Vitamin D Status. The Journal of Clinical Endocrinology & Metabolism, 91(8), 3055–3061. Added by: Sarina (2022-08-05 13:09:32) |
| Resource type: Journal Article DOI: 10.1210/jc.2006-0710 ID no. (ISBN etc.): 0021-972X BibTeX citation key: Singh2006 View all bibliographic details  |
Categories: Englisch = English Creators: Grebe, Reddy, Singh, Taylor Collection: The Journal of Clinical Endocrinology & Metabolism |
Views: 3/133 Views index: % Popularity index: 0.5% |
| Abstract |
|
{Context: We have recently introduced liquid chromatography-tandem mass spectrometry (LC-MS/MS) for 25-hydroxyvitamin D2 (25OHD2) and 25OHD3 testing. During subsequent clinical use, we identified significantly elevated results in some infants. We hypothesized this might represent assay interference caused by C-3 epimers of 25OHD2 or 25OHD3.Objective: Our aims were to 1) determine the prevalence of C-3 epimers of 25OHD2 or 25OHD3 in human serum, and 2) identify the patient populations that might be affected.Study Design: We modified our LC-MS/MS method to allow detection of C-3 epimers. We retested specimens from four patient groups with the new method and an extracted RIA: 1) children less than 1 yr old, 2) children 1–18 yr old, 3) adults aged 20–87 yr with liver disease, and 4) adults aged 19–91 yr without liver disease.Results: In 172 children from group 1 with detectable 25OHD2 or 25OHD3, we identified C-3 epimers in 39 (22.7%). The epimers contributed 8.7–61.1% of the total 25-OHD. There was an inverse relationship between patient age and epimer percentage (r = 0.48; P < 0.002). The RIA gave accurate 25-OHD results that correlated with the modified LC-MS/MS method. No C-3 epimers were detected in any of the other groups.Conclusions: Significant concentrations of C-3 epimers of 25OHD2 or 25OHD3 are commonly found in infants. This can lead to overestimation of 25-OHD levels. Measurements in children less than 1 yr should therefore be performed with an assay that allows accurate detection of 25-OHD in the presence of its C-3 epimers.}
Added by: Sarina |